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Figure 1 | BMC Proceedings

Figure 1

From: Development of a mammalian cell culture process for rapid Clinical-Scale production of novel Influenza Nanoparticle vaccines

Figure 1

A schematic representation of the transient transfection process is shown. The HEK 293 cells were grown in a 50L Single Use Bioreactor (SUB). Cells were then washed and concentrated using a semi continuous centrifuge (kSep) and harvested into 3 Wave bags. Transfection was carried out in the 3 Wave bags by adding pDNA and PEI. After 2 hours of incubation, production media was added. To facilitate expression, Valproic acid (VPA) and production feed were added at 24 and 48 hours post-transfection, respectively and culture was harvested at 96 hours post-transfection.

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