Figure 1
From: Purification of modified vaccinia virus Ankara from suspension cell culture
Prevention of copurification of host-cell derived DNA. (a) Enveloped virions appear to associate stably with host cell DNA. Chaotropes were added prior to lysis to prevent complex formation and to allow DNase to access its substrate without sterical hindrance. (b) A representative chromatogram of a large pore CIM ion exchange column loaded with lysate of MVA-infected CR.pIX cells. Elution fraction A is designed to remove DNA, the infectious units collect in B. (c) Quantification of the ratios of viral to cellular DNA by qPCR. The ratios of the load were set to 1. Blue columns show values obtained in presence and green in absence of chaotropes. (d) Quantification of yields relative to vaccine dose for a process with chaotropes.