Identification of process parameters influencing product quality in mammalian cell culture

BMC Proceedings

Table 1 Cell culture parameters influencing cell growth, mAb productivity and protein aggregation.

	Cell concentration [Cells/mL]		mAb productivity [pg/Cell × day]		FDBA assay [RFU]		Particle formation [NC/U LDH]
Parameters	Mock	Producer	Mock	Producer	Mock	Producer	Mock	Producer
pH value	0	0	n.a.	0	0	0	0	0
Osmolality	-	-	n.a.	+	0	0	-	-
Agitation	0	0	n.a.	0	+	+	+	+
VPA	0	-	n.a.	+	0	0	0	+
Antifoam	0	0	n.a.	0	0	0	0	0

Result of the DoE screening: (+) Positive effect on response variable, (-) negative effect on response variable, (0) no effect on response variable, (n.a.) not analyzed.

ISSN: 1753-6561