Figure 1

Impact of manganese concentration in cell culture media on fucosylation, mannosylation and CD16 binding affinity of produced monoclonal antibodies. A linear dependency of CD16 binding affinity (empty circles) and fucosylation (filled triangles) with manganese levels was characterized in 14-day fed-batch experiments in 15 mL-scale bioreactors (a). Mannosylation (filled diamonds) did not correlate with manganese concentrations in a strictly linear manner. Results from the screening experiment were verified with a different cell line in 2 L- scale (b). Final harvest titers (filled bars) were not impacted by manganese additions, while CD16 binding affinity of the product could be doubled (striped bars) by reducing fucosylation levels (dotted bars) by 10 % with 150 µM manganese as media supplement. Error bars indicate the standard deviation of biological duplicates.