Figure 1
From: Generation and characterization of a superior host cell line for biomanufacturing
(a): Comparison of productivities of an Fc-fusion protein after transient transfection. Average of 3 pools ± STDev.(b): Viabilities after transfection (day 0) and during selection for three CHO-C8DEL cell lines (green) compared to the CHO-K1a cell line (blue) expressing a monoclonal antibody. Average of 4 pools ± STDev. (c): After both selection steps a massive increase of productivity of a monoclonal antibody could be detected for all three CHO-C8DEL lines (green) compared to the CHO-K1a cell line (blue). Average of 4 pools ± STDev.(d): After single cell cloning of high producing pools of CHO-C8DEL 3 and CHO-K1a, significantly more high producing cells were isolated for the CHO-C8DEL 3 cell line. E: Productivity of 45 best producing CHO-K1a and CHO-C8DEL 3 clones. F: Comparison of stability of 45 best producing CHO-C8DEL 3 clones compared to 37 best producing CHO-K1a clones over a cultivation period of 12 weeks.