From: Reconciling pillars of transient gene expression: From DNA prep via media, reagent and cell line development to holistic process optimization
Pseudo-perfusion
Batch
Fed-Batch
Viable cell density
40 x106 cell/mL
5 x106 cell/mL
20 x106 cell/mL
Volume [mL]
10
30-3000
30-300
Duration [days]
7
6
Yield [mg/L]
240*
400
850