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Table 1 Transient expression of a monoclonal antibody in independent experiments with differing setups and scales.

From: Reconciling pillars of transient gene expression: From DNA prep via media, reagent and cell line development to holistic process optimization

 

Pseudo-perfusion

Batch

Fed-Batch

Viable cell density

40 x106 cell/mL

5 x106 cell/mL

20 x106 cell/mL

Volume [mL]

10

30-3000

30-300

Duration [days]

10

7

6

Yield [mg/L]

240*

400

850

  1. * final antibody concentration in perfundate