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Table 1 Recovery of total and infectious viral particles and removal of total proteins in purification steps in a 3 L scale run.

From: Optimization and scale-up of cell culture and purification processes for production of an adenovirus-based tuberculosis vaccine

Samples Total viral particles Recovery Total infectious particles Recovery Total proteins (mg) Recovery
Harvest broth 7.1 × 1013 100% 6.5 × 1012 100% 1112 100%
10X conc. lysate 5.1 × 1013 72% 5.7 × 1012 88% 993 89%
Q-Seph HP feed 7.2 × 1013 101% 5.0 × 1012 77% 882 79%
Q-Seph HP
1M NaCl peak
5.3 × 1013 75% 3.8 × 1012 59% 68 6%
Capto Core 700 flowthrough 4.3 × 1013 61% 3.2 × 1012 49% 11 1%
Final product 2.0 × 1013 28% 2.6 × 1012 40% 4 0.4%