Skip to main content

Table 1 Recovery of total and infectious viral particles and removal of total proteins in purification steps in a 3 L scale run.

From: Optimization and scale-up of cell culture and purification processes for production of an adenovirus-based tuberculosis vaccine

Samples

Total viral particles

Recovery

Total infectious particles

Recovery

Total proteins (mg)

Recovery

Harvest broth

7.1 Ă— 1013

100%

6.5 Ă— 1012

100%

1112

100%

10X conc. lysate

5.1 Ă— 1013

72%

5.7 Ă— 1012

88%

993

89%

Q-Seph HP feed

7.2 Ă— 1013

101%

5.0 Ă— 1012

77%

882

79%

Q-Seph HP

1M NaCl peak

5.3 Ă— 1013

75%

3.8 Ă— 1012

59%

68

6%

Capto Core 700 flowthrough

4.3 Ă— 1013

61%

3.2 Ă— 1012

49%

11

1%

Final product

2.0 Ă— 1013

28%

2.6 Ă— 1012

40%

4

0.4%