Table 1 Summary of cell establishment and characterization.
| Â | Clone1 | Clone2 | Clone3 |
|---|---|---|---|
Culture medium for transformation | 12.5%FBS-ERDF +IL-4+IL-6+PMA | 12.5%FBS-ERDF +IL-6+PMA | 12.5%FBS-ERDF +IL-4+IL-6+PMA |
Culture medium for maintenance | 12.5%FBS-ERDF (+PMA) | 12.5%FBS-ERDF (+PMA) | 12.5%FBS-ERDF (+PMA) |
Possible period of subculture | Over 1 year | Over 1 year | Over 2 years |
Mean doubling time | 30 hours | 30 hours | 30 hours |
TERT expression | Positive | Positive | Positive |
Produced Immunoglobulin | IgG | IgM | IgG |
B cell marker (CD19) | Positive | Positive | Positive |