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Figure 1 | BMC Proceedings

Figure 1

From: Characterization of Alternative Promoters to Stagger and Control Protein Expression in the Baculovirus-Insect Cell System: From Intracellular Reporter Proteins to Fluorescent Influenza Virus-like Particles

Figure 1

(a) Uniform fluorescence in cells infected with baculovirus carrying GFP and RFP under two different promoters. (b) Localized protein expression in cells infected with baculovirus carrying HAGFP and M1RFP under two different promoters. (c) Smoothed flow cytometry scatterplots of iodixanol gradient purified VLP samples. Left scatterplot is the analysis of material recovered from the supernatant of insect cell cultures infected with a baculovirus having the HAGFP gene under the control of the p10 promoter and M1RFP under the control of the polh promoter. Right scatterplot is the analysis of material recovered from the supernatant of insect cell cultures infected with a baculovirus having the HAGFP gene under the control of the vcath promoter and M1RFP under the control of the polh promoter. (d) TEM image of VLPs produced from cells infected with a baculovirus having the HAGFP gene under the control of the p10 promoter and M1RFP under the control of the polh promoter.

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