Table 1 Summary of obtained results for uncoated (PS),wetted (PBS) or coated surfaces by XPS analysis of surface chemical composition1, contact angles measurements of surface wettability2, HPLC dosages of remaining peptides in solution after coating3, hADSCs adhesion efficiencies assessment(6h after seeding)4and hADSCsPDL determination after 5 days of culture5.
From: Synthetic peptide matrices as support for stem cells culture
XPS 1 O/C (N = 3, n = 3) | Contact angles (°) 2 (N = 3, n = 15) | HPLC (% of remaining peptides) 3 (N = 2, n = 3) | Adhesion (% ofadherent cells) 4 (N = 3, n = 3) | Growth (PDL) 5 (N = 3, n = 3) | ||
|---|---|---|---|---|---|---|
PS (control) | (124 ± 12) × 10-3 | (7 ± 3) × 10-3 | 81.5 ± 0.6 | / | / | / |
PBS (control) | / | / | 60.3 ± 2.3 | / | 49.7 ± 2.0 | 1.76 ± 0.08 |
BSA | / | / | 51.4 ± 3.2 * | / | 9.6 ± 1.0* | < 0* |
Collagen I | / | / | / | / | 63.1 ± 1.6* | 2.12 ± 0.04 |
Fibronectin | / | / | / | / | 44.1 ± 1.1 | 1.90 ± 0.04 |
Puramatrix® | / | / | / | / | 47.9 ± 2.3 | 1.66 ± 0.09 |
P1 | (174 ± 18) × 10-3 | (44 ± 3) × 10-3* | 35.5 ± 0.8* | 71.6 ± 5.5 | 61.6 ± 1.9* | 2.13 ± 0.06 |
P2 | (204 ± 13) × 10-3 | (27 ± 5) × 10-3 | 25.8 ± 1.5* | 87.9 ± 2.3 | 66.7 ± 2.1* | 1.80 ± 0.03 |
P3 | (282 ± 47) × 10-3* | (63 ± 10) × 10-3* | 19.6 ± 0.7* | 63.8 ± 4.8 | 69.2 ± 2.3* | 1.90 ± 0.08 |
P4 | (141 ± 13) × 10-3 | (55 ± 13) × 10-3* | 14.8 ± 0.8* | 51.2 ± 4.7 | 54.8 ± 2.0 | 2.07 ± 0.08 |