Table 1 Classification power of "unknown" samples(up to eight technical replicates per biological sample) from perfusion mode using the CPT model built with spectral information from samples cultivated under batch mode. #V: number of technical replicates classified as viable; #A/N: number of technical replicates classified asapoptotic/ necrotic.
From: MALDI-TOF mass spectrometry biotyping: At line monitoring of recombinant CHO cell cultures
"unknown" sample | Drop of viability detected by Trypan blue | Apoptosis assessment by activity assessment of Caspase 9 | Result of unsupervised classification based on spectral information | Mean Positive Predictive Value (PPV) | |
|---|---|---|---|---|---|
[h] | [Y/N] | [Y/N] | #V | #A/N | [%] |
24 | N | N | 6 | 2 | 78 |
48 | N | N | 5 | 1 | Â |
72 | N | N | 5 | 1 | Â |
96 | N | N | 6 | 1 | Â |
120 | N | N | 4 | 3 | Â |
144 | N | N | 6 | 1 | Â |
152 | N | Y | / | 8 | 100 |
160 | N | Y | / | 6 | Â |
168 | Y | Y | / | 7 | Â |
192 | Y | Y | / | 6 | Â |