Table 1 Summary of Allegro XRS 20 Bioreactor operating conditions.
XRS 20 Bioreactor | Conventional Rocking Bioreactor | |
|---|---|---|
Start Agitation (5 L) | ||
• Rate (CPM) | 25 | 25 |
• X Angle (Deg) | 5 | N/A |
• Y Angle (Deg) | 5 | 8 |
Agitation (14 - 20 L) | ||
• Rate (CPM) | 35 | 42 |
• X Angle (Deg) | 5 | N/A |
• Y Angle (Deg) | 15 | 10 |
Aeration Rate (L/min) | 1.0 | 1.0 |
Seed Density (106 Cells/mL) | 0.3 ± 0.1 | 0.3 ± 0.1 |
pH Set Point | 7.2 | 7.2 |
Do Set Point (%) | 40 | 40 |
Glucose Concentration (g/L) | >2.0 | >2.0 |