Figure 1
From: PAIA assays: A new bead-based assay system for high throughput protein quantification
PAIA Human IgG Fc/Fab assay.(a) Description of the PAIA assay workflow: Each of the wells of the 384 well PAIA plate contains pre-dispensed dried capture beads. The depicted procedure is run in parallel in each of the wells. The unique structure of the plate allows detection of the unbound fluorescence marker only through the transparent protrusion which keeps the beads outside the detection zone and light path. (b) IgG1 and IgG2 quantification in buffer. (c) IgG1 quantification in cell culture media. It is recommended to prepare standards and samples in the same matrix. Medium 1: Gibco Freestyle CHO + 8 mM Glutamax, Medium 2: BD Select CHO + 8 mM L-Ala-L-Gln, Medium3: Biochrome ISF-1 + 10% FBS. (d) IgG1 calibration curves for several sample volumes. The dynamic range is adapted to higher (2 μL) or lower (10 μL) IgG concentrations by the variation of sample volume.