Ex vivo production of red blood cells from human cord blood

BMC Proceedings

Table 1 Summary of kinetic parameters.

	MANUAL CULTURE	BIOREACTOR CULTURE
Enucleation percentage	44.20%	67.50%
Initial CD34 ⁺ cell number	2x10⁵	5x10⁵
Total Expansion Factor	7.27x10⁴	1.99x10⁴
Theoretical number of total cells	1.45x10¹⁰	9.95x10⁹
Theoretical number of total enucleated cells	6.41x10⁹	6.72x10⁹
Theoretical number of enucleated cells per CD34 ⁺	3.2x10⁴	1.34x10⁴

Results at day 21, from one out of 3 independent experiments. Theoretical values indicate the predicted values if media volumes were increased instead of fixed at 250 mL.

ISSN: 1753-6561