Figure 1From: RNAi-mediated knockdown of Dnmt3a enhances antibody titer in CHO cells up to 200 percentCorrelation between enhanced antibody production and decreased Dnmt3a expression level. (a) Correlation between cell specific productivity of a CHO-XL99 clone in cultivation time course of perfusion and Dnmt3a protein expression level (n = 4, protein expression levels were normalized to t6). (b) Average monoclonal antibody concentrations of transient transfected CHO-XL99 cells and untransfected control, n = 2. Numbers above bars represent cell specific productivities [pg/(cell × d)] within 24 h. (c) Average viable cell densities of transfected CHO-XL99 cells and untransfected control. (d) Average viabilities of transfected CHO-XL99 cells and untransfected control.Back to article page