Fig. 1 (abstract P-087).

Reduction of experimental space and comparison of simulated and experimental medium optimization, experimental design was done in Design Expert 9 (I-optimal Design, 5 lack-of-fit, 5 replicate points)); b model was fitted to mean of 4 parallel shaking flask cultivations (80 ml working volume, MATLAB 2015a), c cultivation was done in 16 parallel shaking flasks (40 mL working volume each), Incubator: Kuhner LT-XC (37°C, 5% CO2, 85% humidity, 12,5 mm shaking diameter, 200 rpm), Medium: TC-42, Xell AG