Fig. 1 (abstract P-294).
CHO GS-/- cells were transfected with 4 different sets of expression plasmids: Reference (without 2GUN; grey), 3-vector 2GUN (green), 2-vector 2GUN with γ-α (pink), and 2-vector 2GUN with α-γ (orange). Panel a: recovery of bulk pools on selective medium. Each line is the average of 4 bulk pools: 2 replicate transfections, each split into two flasks after transfection. Mock and GFP transfected pools didn’t recover (light grey lines). Panel b: After recovery, each pool was seeded in fresh medium in shaker flasks and cultured for 9 days. The bars show the average expression of 4 bulk pools