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Table 1 (abstract P-004). PEIpro®, the reagent of choice for virus production runs in most cell culture systems in both adherent and suspension cells. Irrespective of the cell culture-based system and production scale, PEIpro® and PEIpro®-HQ have led to efficient viral vector yields superior to 107 IG/mL and 109 VG/mL, respectively for lentiviruses and AAVs

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Cell culture system

Vector

Cells

Titer

CS10®/CF10®

AAV

Adherent HEK-293, HEK-293T

1011-1013 VG/ml

Fixed-bed bioreactor (iCELLIS®)

AAV

Adherent HEK-293T

1014-1016 VG/ml

Shaker Flask

AAV

Suspension HEK-293, HEK-293T

109-1010 VP/ml

Bioreactor

AAV

Suspension HEK-293, HEK-293T

0.8-1.5 x109-1010 VG/ml

10 cm dish/75 cm2

Lentivirus

Adherent HEK-293, HEK-293T

1-2 x108 TU/ml

HYPERflask®/HYPERstack®

Lentivirus

Adherent HEK-293, HEK-293T

1-2 x108 TU/ml

Shaker Flask

Lentivirus

Suspension HEK-293F, HEK-293T

2x 107-1010 VP/ml

Bioreactor

Lentivirus

Suspension HEK-293, HEK-293T

107 IG/ml