Skip to main content

Table 1 (abstract P-227). Summary of transfection conditions

From: Abstracts from the 25th European Society for Animal Cell Technology Meeting: Cell Technologies for Innovative Therapies

Parameters

Media screen for GFP transfection

Optimize of GFP transfection

Comparison of transfection method

Media type

ProCHO5, Opti-MEM, CHOMACS CD, ProCHO4, UltraCHO, DMEM/F12

Opti-MEM + CHOMACS CD

Opti-MEM + CHOMACS CD

Transfection volume (w/o or w media addition at 5 hpt*)

5 mL

12.5 + 12.5 mL

2.5 + 2.5 mL

Transfection protocol

Insitu

Insitu

Conventional

Insitu

Cells amount

10 x 106

62 x 106

10 x 106

pEGFP-N1 plasmid pDNA (μg)

6

37.5

5

6

Linear PEI 25 Kda (μg)

30

187.5

15

30

Scale

Spin tube®50 bioreactor

25 mL-Shake flask

Spin tube ®50 bioreactor

  1. *w/o= without and w= with
  2. Transfection efficiency was monitored at 48 hpt by fluorescence microscope and flow cytometer. Cell count and viability were determined by Cedex analyzer