Fig. 3

Leydig cell culture at days 2, 4 and 7. Black arrows indicate Leydig cells. a. The appearance of Leydig cells in culture 24–48 h: Leydig cells are still less. Leydig cells that have grown in polygonal form, b. The appearance of Leydig cells in culture in 96 h. Leydig cells are increasing in number. c Leydig cell appearance in 156 h. Leydig cells have proliferated rapidly and reached confluent of about 80%. Culture was observed using an inverted microscope at 100x magnification