Fig. 1 (abstract P-112).

CRISPR-based targeted integration of hIFNγ transgene into HEK293 cells. HEK293 N1D8 cell line with single eGFP insertion into a chromosomal hotpost (A) was transfected with hIFNγ expression vector with 20bp homology arms together with a CRISPR expression vector containing anti-eGFP sgRNA (B). Clones were isolated and correct knock-in of hIFNγ gene in the desired spot was checked (C)