Fig. 1 (abstract P-561).

Fast workflow for perfusion media development. After initial definition of optimal basal media, eight different feed supplements were screened, optimized, and tested in four consecutive steps (A.). In step 2, a DoE with small-scale semi-continuous perfusion models was applied to optimize the pre-selected feed supplements and to establish valid regression models (B.). The novel media was applied to a perfusion bioreactor (C.)