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Fig. 1 (abstract P-565). | BMC Proceedings

Fig. 1 (abstract P-565).

From: Abstracts from the 26th European Society for Animal Cell Technology Meeting - Cell culture technologies: bridging academia and industry to provide solutions for patients

Fig. 1 (abstract P-565).

A) Cultivation of mAb-producer CHO cells w/o and with IGF supplementation at day 3 without glutamine (left) and with glutamine (right). B) Western blot to present ERK1/2 and AKT protein level over time past IGF induction in cultures with and w/o glutamine. C) Relative quantification of changes in phosphorylation of AKT and ERK1/2 utilizing phospho-specific antibodies in western blots. Signals were normalized to total loaded protein amount via Coomassie-stained membrane. D) Triple SILAC-MS data of replicate 1 evaluated by Proteome Discoverer software filtered for ERK quantification as time course past IGF induction with and without glutamine. ERK2 protein level (left) and ERK2 peptide expression data as unphosphorylated peptide and double phosphorylated variant (right). E) Significantly regulated phosphopeptides for one selected time point (5 min past IGF induction) with or without glutamine

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