Fig. 1 (abstract P-591).
Schematic representation of the expressed CD19-AD2 fusion construct and evaluation of T cells fluxing Ca2+ for determination of the biological activity of CD19-AD2. Therefore, a planar glass-supported lipid bilayer (SLB) was functionalized with the adhesion molecule ICAM-1, the costimulatory molecule B7-1 and CD19-AD2 for recognition by CAR-T cells [2]. The proportion of Ca2+ signaling cells at two different CD19-AD2 densities on the SLB was measured. As negative control, cells were additionally confronted with antigen-free SLB presenting only ICAM-1 and B7-1