Volume 7 Supplement 6

23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health

Meeting abstracts

Edited by Hansjörg Hauser

Publication charges for this supplement were funded by the European Society for Animal Cell Technology (ESACT). The Supplement Editor declares that he has no competing interests.

23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health.

Lille, France23-26 June 2013

Concatenated PDF 7S6

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Engineering characterisation of single-use bioreactor technology for mammalian cell culture applications

Authors: Akinlolu Odeleye, Gary J Lye and Martina Micheletti

Citation: BMC Proceedings 2013 7(Suppl 6):P91

Content type: Poster presentation Published on: 4 December 2013
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Enhancing cell growth and antibody production in CHO cells by siRNA knockdown of novel target genes

Authors: Sandra Klausing, Oliver Krämer and Thomas Noll

Citation: BMC Proceedings 2013 7(Suppl 6):P92

Content type: Poster presentation Published on: 4 December 2013
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Skin and hair-on-a-chip: Hair and skin assembly versus native skin maintenance in a chip-based perfusion system

Authors: Ilka Wagner, Beren Atac, Gerd Lindner, Reyk Horland, Matthias Busek, Frank Sonntag, Udo Klotzbach, Alexander Thomas, Roland Lauster and Uwe Marx

Citation: BMC Proceedings 2013 7(Suppl 6):P93

Content type: Poster presentation Published on: 4 December 2013
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2D fluorescence spectroscopy for real-time aggregation monitoring in upstream processing

Authors: Karen Schwab and Friedemann Hesse

Citation: BMC Proceedings 2013 7(Suppl 6):P94

Content type: Poster presentation Published on: 4 December 2013
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Use of microcarriers in Mobius^® CellReady bioreactors to support growth of adherent cells

The Mobius^® CellReady bioreactor product platform incorporates novel disposable technologies that provide optimal performance for suspension mammalian cell culture. Here we show the utility of EMD Millipore's 3L ...

Authors: Michael McGlothlen, Donghui Jing, Christopher Martin, Michael Phillips and Robert Shaw

Citation: BMC Proceedings 2013 7(Suppl 6):P95

Content type: Poster presentation Published on: 4 December 2013
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CHO starter cell lines for manufacturing of proteins with pre-defined glycoprofiles

Authors: Karsten Winkler, Michael Thiele, Rita Berthold, Nicole Kirschenbaum, Marco Sczepanski, Henning von Horsten, Susanne Seitz, Norbert Arnold, Axel J Scheidig and Volker Sandig

Citation: BMC Proceedings 2013 7(Suppl 6):P96

Content type: Poster presentation Published on: 4 December 2013
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Dynamic profiling of amino acid transport and metabolism in Chinese hamster ovary cell culture

Authors: Sarantos Kyriakopoulos, Karen M Polizzi and Cleo Kontoravdi

Citation: BMC Proceedings 2013 7(Suppl 6):P97

Content type: Poster presentation Published on: 4 December 2013
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Optimized platform medium and feed for rCHO cell lines using the CHEF1^® expression system

Authors: William Paul, Raymond Davis, Andrew Campbell, Sarah Terkildsen, Vann Brasher, James Powell, Blake Engelbert and Howard Clarke

Citation: BMC Proceedings 2013 7(Suppl 6):P98

Content type: Poster presentation Published on: 4 December 2013
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Profiling of glycosylation gene expression in CHO fed-batch cultures in response to glycosylation-enhancing medium components

Authors: Ryan Boniface, Jeoffrey Schageman, Brian Sanderson, Michael Gillmeister, Angel Varela-Rohena, John Yan, Yolanda Tennico, Shawn Barrett, Robert Setterquist and Stephen Gorfien

Citation: BMC Proceedings 2013 7(Suppl 6):P99

Content type: Poster presentation Published on: 4 December 2013
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How to assess chemically defined media and feeds from 9 suppliers on CHO cells producing mAb

Authors: Aurore Polès-Lahille, Margaux Paillet, Aurélie Da Silva, Nora Kadi, Eric Basque, Flavien Thuet, David Balbuena and Sébastien Ribault

Citation: BMC Proceedings 2013 7(Suppl 6):P100

Content type: Poster presentation Published on: 4 December 2013
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Evaluation of single-use bioreactors for perfusion processes

Authors: Aurore Polès-Lahille, Flavien Thuet, David Balbuena and Sébastien Ribault

Citation: BMC Proceedings 2013 7(Suppl 6):P101

Content type: Poster presentation Published on: 4 December 2013
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Profiling and engineering of microRNAs for enhancing recombinant protein productivity in Chinese hamster ovary cells

Authors: Wan Ping Loh, Bernard Loo, Lihan Zhou, Peiqing Zhang, Dong Yup Lee, Yuan Sheng Yang and Kong Peng Lam

Citation: BMC Proceedings 2013 7(Suppl 6):P102

Content type: Poster presentation Published on: 4 December 2013
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Designing clinical-grade integrated strategies for the downstream processing of human mesenchymal stem cells

Authors: Bárbara Cunha, Margarida Serra, Cristina Peixoto, Marta Silva, Manuel Carrondo and Paula Alves

Citation: BMC Proceedings 2013 7(Suppl 6):P103

Content type: Poster presentation Published on: 4 December 2013
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Culture supplement extracted from rice bran for better serum-free culture

Authors: Satoshi Terada, Satoko Moriyama, Ken Fukumoto, Yui Okada, Rinaka Yamauchi, Yoko Suzuki, Masayuki Taniguchi, Shigeru Moriyama and Takuo Tsuno

Citation: BMC Proceedings 2013 7(Suppl 6):P104

Content type: Poster presentation Published on: 4 December 2013
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Cryopreservative solution using rakkyo fructan as cryoprotectant

Authors: Satoshi Terada, Shinya Mizui, Yasuhito Chida, Masafumi Shimizu, Akiko Ogawa, Takeshi Ohura, Kyo-ichi Kobayashi, Saori Yasukawa and Nobuyuki Moriyama

Citation: BMC Proceedings 2013 7(Suppl 6):P105

Content type: Poster presentation Published on: 4 December 2013
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Rice bran extract (RBE) as supplement for cell culture

Authors: Satoko Moriyama, Ken Fukumoto, Masayuki Taniguchi, Shigeru Moriyama, Takuo Tsuno and Satoshi Terada

Citation: BMC Proceedings 2013 7(Suppl 6):P106

Content type: Poster presentation Published on: 4 December 2013
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Identification of mitogenic factor in rice bran for better mammalian cell culture

Authors: Yoko Suzuki, Satoko Moriyama, Masayuki Taniguchi, Shigeru Moriyama, Takuo Tsuno and Satoshi Terada

Citation: BMC Proceedings 2013 7(Suppl 6):P107

Content type: Poster presentation Published on: 4 December 2013
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Protein folding and glycosylation process are influenced by mild hypothermia in batch culture and by specific growth rate in continuous cultures of CHO cells producing rht-PA

Authors: Mauricio Vergara, Silvana Becerra, Julio Berrios, Juan Reyes, Cristian Acevedo, Ramon Gonzalez, Nelson Osses and Claudia Altamirano

Citation: BMC Proceedings 2013 7(Suppl 6):P108

Content type: Poster presentation Published on: 4 December 2013
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The combined use of platinum nanoparticles and hydrogen molecules induces caspase-dependent apoptosis

Authors: Takeki Hamasaki, Tomoya Kinjyo, Hidekazu Nakanishi, Kiichiro Teruya, Sigeru Kabayama and Sanetaka Shirahata

Citation: BMC Proceedings 2013 7(Suppl 6):P109

Content type: Poster presentation Published on: 4 December 2013
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Rec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering

Authors: Alfred M Engel, Harald Sobek, Michael Greif, Sebastian Malik, Marco Thomann, Christine Jung, Dietmar Reusch, Doris Ribitsch, Sabine Zitzenbacher, Christiane Luley, Katharina Schmoelzer, Tibor Czabany, Bernd Nidetzky, Helmut Schwab and Rainer Mueller

Citation: BMC Proceedings 2013 7(Suppl 6):P110

Content type: Poster presentation Published on: 4 December 2013
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Accelerating stable recombinant cell line development by targeted integration

Authors: Bernd Rehberger, Claas Wodarczyk, Britta Reichenbächer, Janet Köhler, Renée Weber and Dethardt Müller

Citation: BMC Proceedings 2013 7(Suppl 6):P111

Content type: Poster presentation Published on: 4 December 2013
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Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies

Authors: Bo Liu, Carina Villacres-Barragan, Erika Lattova, Maureen Spearman and Michael Butler

Citation: BMC Proceedings 2013 7(Suppl 6):P112

Content type: Poster presentation Published on: 4 December 2013
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Development and application of an automated, multiwell plate based screening system for suspension cell culture

Authors: Sven Markert, Carsten Musmann and Klaus Joeris

Citation: BMC Proceedings 2013 7(Suppl 6):P113

Content type: Poster presentation Published on: 4 December 2013
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Characterization of recombinant IgA producing CHO cell lines by qPCR

Immunoglobulin A (IgA) mediates a key role in mucosal immunity and is a promising novel immunotherapeutic candidate. However, difficulties in obtaining enough material often hamper in vivo explorations. We have p...

Authors: David Reinhart, Wolfgang Sommeregger, Monika Debreczeny, Elisabeth Gludovacz and Renate Kunert

Citation: BMC Proceedings 2013 7(Suppl 6):P114

Content type: Poster presentation Published on: 4 December 2013
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Data integration methodology that couples novel bioreactor monitoring tools, automated sampling, and applied mathematics to redefine bioproduction processes

Authors: Lisa J Graham, Jeffrey F Breit, Lynn A Davis, Corey C Dow-Hygeland and Brandon J Downey

Citation: BMC Proceedings 2013 7(Suppl 6):P115

Content type: Poster presentation Published on: 4 December 2013
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Multicellular tumor spheroids in microcapsules as a novel 3D in vitro model in tumor biology

Authors: Elena Markvicheva, Daria Zaytseva-Zotova, Roman Akasov, Sergey Burov, Isabelle Chevalot and Annie Marc

Citation: BMC Proceedings 2013 7(Suppl 6):P116

Content type: Poster presentation Published on: 4 December 2013
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23rd European Society for Animal Cell Technology (ESACT) Meeting: Better Cells for Better Health

Meeting abstracts

Engineering characterisation of single-use bioreactor technology for mammalian cell culture applications

Enhancing cell growth and antibody production in CHO cells by siRNA knockdown of novel target genes

Skin and hair-on-a-chip: Hair and skin assembly versus native skin maintenance in a chip-based perfusion system

2D fluorescence spectroscopy for real-time aggregation monitoring in upstream processing

Use of microcarriers in Mobius^® CellReady bioreactors to support growth of adherent cells

CHO starter cell lines for manufacturing of proteins with pre-defined glycoprofiles

Dynamic profiling of amino acid transport and metabolism in Chinese hamster ovary cell culture

Optimized platform medium and feed for rCHO cell lines using the CHEF1^® expression system

Profiling of glycosylation gene expression in CHO fed-batch cultures in response to glycosylation-enhancing medium components

How to assess chemically defined media and feeds from 9 suppliers on CHO cells producing mAb

Evaluation of single-use bioreactors for perfusion processes

Profiling and engineering of microRNAs for enhancing recombinant protein productivity in Chinese hamster ovary cells

Designing clinical-grade integrated strategies for the downstream processing of human mesenchymal stem cells

Culture supplement extracted from rice bran for better serum-free culture

Cryopreservative solution using rakkyo fructan as cryoprotectant

Rice bran extract (RBE) as supplement for cell culture

Identification of mitogenic factor in rice bran for better mammalian cell culture

Protein folding and glycosylation process are influenced by mild hypothermia in batch culture and by specific growth rate in continuous cultures of CHO cells producing rht-PA

The combined use of platinum nanoparticles and hydrogen molecules induces caspase-dependent apoptosis

Rec. ST6Gal-I variants to control enzymatic activity in processes of in vitro glycoengineering

Accelerating stable recombinant cell line development by targeted integration

Differential affects of low glucose on the macroheterogeneity and microheterogeneity of glycosylation in CHO-EG2 camelid monoclonal antibodies

Development and application of an automated, multiwell plate based screening system for suspension cell culture

Characterization of recombinant IgA producing CHO cell lines by qPCR

Data integration methodology that couples novel bioreactor monitoring tools, automated sampling, and applied mathematics to redefine bioproduction processes

Multicellular tumor spheroids in microcapsules as a novel 3D in vitro model in tumor biology

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