Volume 5 Supplement 7
Micropropagation of Pinus taedaL. via axillary buds
© Francisdo de Oliveira et al; licensee BioMed Central Ltd. 2011
Published: 13 September 2011
Pinus taeda stands for productivity and quality of its timber . Researches using biotechnology are of great importance and have been applied to the improvement of its timber and plantation . The main method of Pinus propagation is by seeds, once the minicuttings depends on the season of the year or depends of juvenile material [3–5]. Thus, researches on micropropagation of Pinus taeda are currently a priority in Brazil . Micropropagation is the best method for mass production of superior genotypes and represents a strategy for tree improvement and capture of genetic gains . Studies on Pinus taeda micropropagation by axillary bud proliferation are quite few. The purpose of this study was to develop a protocol for micropropagation of this species from juvenile material.
Materials and methods
For in vitro establishment two to four month old seedlings were used. Apical shoots and nodal segments of 3 cm length were inoculated in MS , DCR , WV3  or WV5  medium. For axillary shoots induction, the explants were inoculated in WV3, WV5 or DCR medium, with BAP (0, 0.12, 0.25 and 0.50 μM). For the induction of roots, we tested the effect of double-layer medium, with semi-solid phase consisting of agar and water or GDm/2  medium and the liquid phase containing water or GDm/2 medium. Both phases were supplemented with 2.69 μM NAA and 0.44 μM BAP for 9 days, followed by transfer to growth regulator-free GDm/2 medium. The rooted plants were planted in Plantmax® Forestry substrate and maintained in a greenhouse.
Results and Discussion
The autors are grateful to Battistella Florestal Company for the financial assistance, to FINEP (Financiadora de Estudos e Projetos), to REUNI (Reestruturação e Expansão das Universidades Federais) and the Federal University of Paraná, Brazil.
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