Volume 5 Supplement 8
Anti-diabetes effect of water containing hydrogen molecule and Pt nanoparticles
© Shirahata et al; licensee BioMed Central Ltd. 2011
Published: 22 November 2011
Electrochemically reduced water (ERW) contains a lot of hydrogen molecule (H2) and scavenges reactive oxygen species (ROS) to protect DNA from oxidative damage . ERW also contains small amounts of Pt nanoparticles (NPs) and elongates the lifespan of C. elegans . Pt NPs are newly recognized multi-functional ROS scavengers . ERW exhibits anti-diabetes effects in vitro and in vivo [4–6]. We proposed mineral nanoparticle active hydrogen reduced water hypothesis to explain the activation mechanism of H2 to hydrogen atom (H). Recently, H2 has been reported to scavenge ROS and suppress a variety of oxidative stress-related diseases , however, the action mechanism of H2 has not been clarified thoroughly. Here, we examined anti-diabetes effects of H2 and Pt NPs.
Materials and methods
Pt NPs of 2-3 nm sizes were synthesized from H2PtCl6 by the citrate reduction method. L6 rat myoblast cells (1.2 x 105 cells) were inoculated into a 35 mm culture dish and a day later, the cells were treated with or without 25mM N-acetylcystein in the presence of BES-H2O2, a H2O2-specific detection reagent in DMEM for 2 h. After washing the cells, molecular hydrogen treatment was performed in a dark condition by cultivating cells in a fresh DMEM medium in a mixed gas incubator under an atmosphere of 75%N2/20%O2/5%CO2 or 75%(H2 and N2 mixed gas)/20%O2/5%CO2 for 1.5 h, followed by flowcytometric analysis. In this condition, culture medium contained maximum 0.4-0.5 ppm of dissolved hydrogen. Glucose uptake of differentiated myotube L6 cells was examined after treating the cells with 3H-2-deoxyglucose for 10 min. Gene expression of catalase (CAT), glutathione peroxidase (GPx) and hemoxoigenase (HO-1) was examined using RT-PCR method. Three weeks old type 2 diabetes model mice (KK-A y ) were fed H2 and/or Pt Nps-containing water ad lib for 6 weeks.
H2, Pt NPs, and their combined use resulted in activation of glucose uptake signal transduction pathways and stimulation of glucose uptake into L6 myotubes. In the groups of H2, Pt NPs and their combined use groups, blood sugar levels and impaired sugar tolerance of type 2 diabetes model mouse (KK-A y ) were significantly improved, suggesting that H2, Pt NPs and H are redox regulation factors in animal cells.
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