Volume 6 Supplement 3

Metabolism, diet and disease

Open Access

Expression of the glucose transporters GLUT1, GLUT3, GLUT4 and GLUT12 in human cancer cells

  • Carly Barron1,
  • Evangelia Tsiani1 and
  • Theodoros Tsakiridis2, 3
BMC Proceedings20126(Suppl 3):P4

DOI: 10.1186/1753-6561-6-S3-P4

Published: 1 June 2012


It is well known that cancer cells display increased glucose uptake and consumption [1]. In a rate-limiting step for glucose metabolism, the glucose transporter (GLUT) proteins facilitate glucose uptake across the plasma membrane. In human cells, 14 different GLUT (1-14) isoforms have been discovered. Of these, GLUT1, 3 and 4 have been extensively studied while the more recently discovered GLUT12 has high relevance to cancer [2]. A comprehensive view of the basal levels of GLUT proteins in cancer is lacking and the exact role of these proteins in cancer cell metabolism is unclear. The present study examined GLUT expression in a number of human cancer cell lines.

Materials and methods

Real-time PCR was used to quantify mRNA levels of GLUT1, GLUT3, GLUT4 and GLUT12 in lung (A549, NCI-H460, SK-MES-1, H1299), breast (MDA-MB-231, MCF-7), and prostate (PC-3, 22RV1, LNCaP) cancer cells and the normal epithelial cells MRC-5, 184B5 and PNT1A. Protein expression of the glucose transporters was examined by immunoblotting and fluorescence microscopy. The expression of GLUT1 and GLUT3 was immunohistochemically examined in tumors from Balb/c nude mice xenografted with A549, H1299 and PC-3 human cancer cells.


GLUT1, GLUT3, GLUT4 and GLUT12 mRNA was detected at various levels in all cells examined. Cancer cells were found to have significantly higher expression of GLUT proteins than the corresponding normal epithelial cells at both the mRNA and protein level. Furthermore, GLUT expression in cancer was found to follow an abnormal expression pattern compared to healthy cells. GLUT1 and GLUT3 were detected in vivo in human cell line xenograft tumors.


These findings describe GLUT expression in a number of cancer cells with different mutations and histologies. The differences in the levels and patterns of glucose transporter expression between normal and cancerous human epithelial cells suggests GLUT proteins may serve as an attractive target for the development of therapeutic agents in lung, breast and prostate cancer.

Authors’ Affiliations

Department of Community Health Science, Brock University
Translational Radiation Biology Laboratory, Juravinski Cancer Centre
Department of Oncology, McMaster University


  1. Ganapathy V, Thangaraju M, Prasad PD: Nutrient transporters in cancer: Relevance to Warburg hypothesis and beyond. Pharmacol Ther. 2009, 121 (1): 29-40.PubMedView ArticleGoogle Scholar
  2. Rogers S, Docherty SE, Slavin JL, Henderson MA, Best JD: Differential expression of GLUT12 in breast cancer and normal breast tissue. Cancer Lett. 2003, 193 (2): 225-233.PubMedView ArticleGoogle Scholar


© Barron et al; licensee BioMed Central Ltd. 2012

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.