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Frequency of Human Papillomavirus infection in squamous cell carcinoma of the oral cavity and oropharynx
BMC Proceedings volume 8, Article number: P67 (2014)
Background
Squamous cell carcinoma of the oral cavity and oropharynx is a public health issue, with over 200,000 new cases worldwide each year [1]. Studies show that the incidence is higher in male individuals, aged between 40 and 55 years. The consumption of tobacco and alcohol are well-established risk factors for developing head and neck cancer, and studies have been shown synergism between these substances, which contributes to the increased risk [4]. Recently, persistent infection with high risk Human Papillomavirus (HPV) has been identified as a potential risk factor for the development of these tumors [2]. HPV is a DNA virus that features tropism to epithelial cells. The high risk HPV types, such as types 16 and 18 have the ability to integrate their DNA into the host cell DNA, immortalizing keratinocytes, with great possibilities of causing a cancer by infecting cells [3]. This study aims to evaluate the frequency of HPV infection, smoking and alcohol consumption related to the development of squamous cell carcinomas of the oral cavity and oropharynx.
Methods
99 frozen tumors samples, classified as squamous cell carcinoma of the oral cavity (n = 83) and the oropharynx (n = 16) were analysed. The polymerase chain reaction was performed from the DNA of the samples, using the Human β-globin genes. After, electrophoresis was performed in 1% agarose gel containing ethidium bromide, to confirm the amplification and quality of extracted DNA, then the DNA samples were subjected to nested PCR with primers MY09/11 and GP5 + / GP6 + for viral DNA detection of HPV subtypes 16 and 18. Samples known to be positive for both subtypes of HPV 16 and 18 were used positive controls. Subsequently, the amplified samples were submitted to electrophoresis on a polyacrylamide gel. History of exposure to tobacco and alcohol was investigated through interviews.
Results and conclusions
Patients with tumors were mostly male (80.81%) aged 30-93 years (average 57.6 years). The use of tobacco was observed in 84.8% of cases and the alcohol abuse of 77.7%. HPV infection was detected in 4 of 99 samples, which corresponds to a low prevalence (4.04%). All of the HPV positive cases also presented the historical of tobacco and alcohol use, that represented about 74.74% of the patients. Studies on the frequency of HPV in Brazilian subjects with head and neck tumors has shown variation 0-33% positivity. [5] Therefore, it is not possible affirm that HPV infection is the main cause of squamous cell carcinoma in these patients, since there is patients that consume alcohol and tobacco concomitantly, which are also risk factors for developing the disease.
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Acknowledgements
CNPq, FAPES, CAPES, PPGBiotec-UFES.
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de Abreu, P.M., Leite Azevedo, P., Gregório Có, A.C. et al. Frequency of Human Papillomavirus infection in squamous cell carcinoma of the oral cavity and oropharynx. BMC Proc 8 (Suppl 4), P67 (2014). https://doi.org/10.1186/1753-6561-8-S4-P67
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DOI: https://doi.org/10.1186/1753-6561-8-S4-P67