Volume 5 Supplement 8

22nd European Society for Animal Cell Technology (ESACT) Meeting on Cell Based Technologies

Open Access

Platform process for production of monoclonal antibodies for research purposes – improvement option

BMC Proceedings20115(Suppl 8):P44

DOI: 10.1186/1753-6561-5-S8-P44

Published: 22 November 2011

Background

In 2006 Novo Nordisk decided to invest in building a pipeline within inflammatory disease management. To support this strategy the cell culture units had to establish technology for expression and production of monoclonal antibodies in CHO cells. A number of technology providers at that time offered proven platform processes for this purpose. It was decided to in-license one of these technology platforms, the one developed at Lonza Biologics, and focus research resources on product innovation rather than development of an in-house production system. The platform has now been fully implemented and the work flow optimised and standardised.

Platform process review

The platform process comprises:

  • Host cell line/expression system

  • Medium/feeds (chemically defined – animal derived component free)

  • Process parameters

  • Scale-down model (shake flask, 100 ml working volume).

The platform process has been applied for cell line development and antibody production for R&D purposes for five years. During this period 11 monoclonal antibodies have been transferred from laboratory scale to pilot plant production. The performance of the process platform across projects has been reviewed. The scope of the review was to compare yields obtained in the scale down model and yields obtained in the bioreactor process for all 11 antibodies. Figure 1 shows the results of the review.
https://static-content.springer.com/image/art%3A10.1186%2F1753-6561-5-S8-P44/MediaObjects/12919_2011_Article_962_Fig1_HTML.jpg
Figure 1

Yields of 11 anitbodies in shake flask and bioreactors.

In conclusion the review show that

  • The cell lines are yielding 1.9 – 4.5 g/l mAb.

  • The scale down model is predictive for the bioreactor process.

Evaluation of an improvement option

An upgrade of the medium, feeds and process protocols was offered by Lonza Biologics. A β-version of the latest process from Lonza has been tested and compared to the previous version in a study including five cell lines. The experiments were carried out in 100 ml shaker flask and the scale down version of the process was applied. A comparison of the two fed batch procedures is shown below:

Current version: Version 6

Medium and feeds CD-ACF

Two feed solution

  • Feed rates based on

   Viable Cell Density

   Residual glucose

New version: Version 8

Medium and feeds CD-ACF

Five feed solutions

  • Feed rates based on

   Viable Cell Density

   Residual glucose

   Time

The results of the study are compiled in table 1.
Table 1

Result of the comparison of the two versions of the fed batch process.

mAb

Current version: Version 6

New version: Version 8

 

ICA*)

Lactate

mAb

ICA

Lactate

mAb

 

10E6 viable cell days/ml

mmol/l

g/l

10E6 viable cell days/ml

mmol/l

g/l

C

108

57

3.5

92

28

7.1

D

103

61

2.2

90

29

5.2

E

162

65

5.5

104

17

9.0

H

100

55

3.2

120

25

7.0

J

148

62

3.2

164

21

4.3

* ) ICA: Integrated Cell Area.

The comparison of the β-test of improved platform process and the current process showed:

  • mAb yields improved 1.3 – 2.4 fold

  • No change in integrated cell area

Improved lactate control may be the key to the yield improvement.

Declarations

Acknowledgements

The following people all contributed with cell lines, data and/or stimulating discussions

Novo Nordisk:Birgitte Friedrichsen, Bjarne Rask Poulsen, Mark Chadfield, Ann Merete Mygind, Ida Mølgaard Kundsen, Max Wellerdiek, Gitte Johnsen.

Lonza Biologics:Alison Porter, Jeetendra Vaghjiani.

Authors’ Affiliations

(1)
Biopharm Manufacturing Development, API Support
(2)
Biopharmaceutical Research, Mammalian Cell Technology

Copyright

© Petersen and Kristensen; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.