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Effect of CCR5 receptor antagonist (MET-RANTES) on leukocyte-endothelium interactions in an experimental model of herpetic encephalitis

  • Márcia de Carvalho Vilela1, 2Email author,
  • Daniel Mansur3,
  • Norinne Lacerda-Queiroz4,
  • David Henrique Rodrigues4,
  • Débora Guerra Amaral2,
  • Graciela Lima3,
  • Erna Kroon3,
  • Marco Antônio Campos3,
  • Mauro Martins Teixeira2 and
  • Antônio Lúcio Teixeira1, 2
BMC Proceedings20082(Suppl 1):P10

Published: 23 September 2008


Herpes SimplexEncephalitisVirus TiterInfected MouseLeukocyte Adhesion


The Herpes Simplex Virus-1 (HSV-1) is responsible for several clinical manifestations in humans, including severe encephalitis. The aim of this study was to use Met-RANTES, a CCR1 and CCR5 antagonist, in the treatment of a severe model of HSV-1 encephalitis.


Wild type C57BL/6 mice were intracerebrally inoculated with 104 PFU of HSV-1 or PBS. Mice received Met-RANTES (10 μg/mice) subcutaneously on days -1, 0, 1, 2 and 3. Visualization of leukocyte recruitment using intravital microscopy was done at 1 and 3 days post-infection (dpi). The brain was removed for chemokine analysis by ELISA. Virus titration in cerebral tissue was done by TCID50.


Met-RANTES does not alter significantly virus titers at 1 dpi and 3 dpi after intracerebral injection of HSV-1. This treatment decreased leukocyte adhesion of microvasculature in C57BL/6 infected mice at 1 and 3 dpi. Treatment was also followed by a significant increase in chemokine levels, including CCL3, CCL5, CXCL1 and CXCL9, at 3 dpi.


CCL5/RANTES, a chemokine that binds to CCR1 and CCR5, seems to be relevant in the recruitment of leucocytes in an experimental model of severe HSV-1 encephalitis. However blocking of CCR1 and CCR5 does not affect HSV-1 replication, suggesting that other immune mechanisms are involved in this process.

Authors’ Affiliations

Departamento de Clínica Médica, Faculdade de Medicina, UFMG, Belo Horizonte, Brazil
Departamento de Bioquímica e Imunologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil
Departamento de Microbiologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Brazil
Departamento de Morfologia, Instituto de Ciências Biológicas, UFMG, Belo Horizonte, Minas Gerais, Brazil


© Vilela et al; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd.