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  • Poster presentation
  • Open Access

Prions hijack tunneling nanotubes for intercellular spread

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  • 1Email author
BMC Proceedings20082 (Suppl 1) :P63

  • Published:


  • Central Nervous System
  • Dendritic Cell
  • Peripheral Nervous System
  • Lymphoid Organ
  • Entry Site

In the infectious variant of Creutzfeldt-Jacobs disease (vCJD), prions (PrPSc) enter the body by oral exposure with contaminated foodstuffs. Prions may then spread from the intestinal entry site to the central nervous system (CNS) by intercellular transfer from the lymphoid system to the peripheral nervous system (PNS). Several mechanisms have been proposed for these intercellular transfer events, including hitch-hiking on membrane-coated viruses, transfer via exosomes or by GPI-painting and different cell types such as dendritic cells, follicular dendritic cells or macrophages have been proposed to be involved. However, the mechanism of cell-to-cell spread remains elusive.

Tunneling nanotubes (TNTs) have recently been identified as a novel means of cell-cell communication both in vitro and in vivo. Here we show that TNTs transfer cellular PrP (PrPc) and PrPSc between cells of the same and different origin. Significantly, we observed fluorescently-labelled PrPSc transferring via TNTs from dendritic cells (DCs) to primary neurons. Since DCs can interact with peripheral neurons in lymphoid organs, TNT mediated intercellular transfer would allow neurons to retrogradely transport prions to the CNS. We propose that TNTs are involved in the spreading of infectious prions from the peripheral site of entry to the PNS by neuroimmune interactions and within the CNS.

Authors’ Affiliations

Unité Trafic Membranaire et Pathogenese, Institut Pasteur, 75015 Paris, France


© Schiff et al; licensee BioMed Central Ltd. 2008

This article is published under license to BioMed Central Ltd.