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Table 1 Identification of hNatB in vitro substrates.

From: Characterization of the human Nα-terminal acetyltransferase B enzymatic complex

PEPTIDE

CPM

PEPTIDE

CPM

BAXA

86

DCUP

51

BRCA1

384

KAD1

502

CASP8

457

B3AT

72

CASP9

7323

CRBA1

119

CDK3

3489

PTN1

2931

CDK8

2665

PPLA

2741

FADD

95

  

TNNC1

82

No-peptide

74

1433E

1763

  

CRYAB

4077

  

NAT2

5363

  

MT1A

1309

  

RS28

1402

  
  1. Immunopurified hNatB complexes with anti-hNaa20p antibody were used to identify 20 amino acids length peptides with Met-Asp- (MD-) or Met-Glu- (ME-) as first two amino acids that are in vitro acetylated by hNatB. The results are representative of at least two independent experiments.