Determination of Km and Vmax for acetyl CoA, based on the generation of acetylated
oligopeptide. Purified MBP-hNaa30p (80 nM) was incubated with 1MLAL-RRR24 oligopeptide at saturated levels and varying concentrations of acetyl CoA (4 – 40 μM) in acetylation buffer for 30 minutes at 37°C. A; Non linear regression analysis of the dose dependent curve generated based on the analysis of the acetylated oligopeptide absorption signal at 215 nm. The coefficient of determination (R2) is given above the plot. B; Hanes-Woolf plot of the dose dependent acetylation signal.