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BMC Proceedings

Open Access

An in vitro tumor-fibroblast interaction model of human oral squamous cell carcinoma

  • Jozséf Dudás1Email author,
  • Mario Bitsche1,
  • Volker Schartinger1,
  • Georg M Sprinzl1 and
  • Herbert Riechelmann1
BMC Proceedings20104(Suppl 2):P32

Published: 24 September 2010


Squamous Cell CarcinomaNeck Squamous Cell CarcinomaPeriodontal LigamentOral Squamous Cell CarcinomaOral Squamous Cell Carcinoma Cell

Carcinoma associated fibroblasts (CAFs) have recently been implicated in neoplastic progression, tumor growth, angiogenesis, and metastasis. This special fibroblast subpopulation plays a major role in initiation of tumor progression of head and neck squamous cell carcinoma.

We established a co-culture model of SCC-25 lingual squamous cell carcinoma cells and periodontal ligament fibroblasts. Gene expression changes were analyzed by real-time PCR, western blot and immunocytochemistry. During 7 days’ co-culture CAFs were converted from normal fibroblasts showing sustained expression of stroma-derived factor. We found that CAFs produced experimentally within such a short time, induced epithelial-mesenchymal transdifferentiation (EMT) of SCC-25 cells in co-culture. EMT-SCC-25 cells showed increased expression of vimentin and the transcription factor snail, and decreased expression of E-cadherin.

These results indicated that CAF-conversion of oral fibroblasts and EMT of oral squamous cell carcinoma cells are coordinated events and could be reliably modeled in vitro.

Authors’ Affiliations

Department of Otorhinology, Medical University of Innsbruck, Innsbruck, Austria


© Dudás et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.