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BMC Proceedings

Open Access

KRAS and EGFR mutations coexisting in lung adenocarcinoma

  • Vitor Sousa1, 2, 3, 4Email author,
  • Ana Alarcão1, 2,
  • Patricia Couceiro1, 2,
  • Maria R Silva1, 3,
  • Maria J d’Aguiar1,
  • Lia Teixeira1 and
  • Lina Carvalho1, 2, 3, 4
BMC Proceedings20104(Suppl 2):P57

Published: 24 September 2010

Lung adenocarcinoma represents about 42% and 28% of NSCLC in women and men. Adenocarcinomas incidence is still rising being the most frequent type of NSCLC diagnosed in USA. Both EGFR and KRAS gene mutations can contribute to the development of NSCLC, namely adenocarcinomas. EGFR and KRAS mutations are considered by some authors as mutually exclusive explained by the fact that KRAS-MAPK pathway is one of the downstream signalling pathways of EGFR. Lung cancers with KRAS mutations are resistant to EGFR tyrosine kinase inhibitors.

Sections of the adenocarcinoma of the lung, formalin-fixed paraffin-embedded tissues (FFPE), were selected to analyze mutations in EGFR exons 19 and 21 and KRAS - codons 12 and 13 by DNA extraction for polymerase chain reaction (PCR). Exon 19 was studied by fragment analysis and exon 21, codons 12 and 13 were studied by direct sequencing. The analysis of FISH results was done by Cappuzzo’s score to EGFR gene. Determination of EGFR protein expression was done by immunohistochemistry (IHC) (Zymed Laboratories).

The authors present a rare case with synchronous EGFR and KRAS mutations. The patient is a 77 years old, male with a central 3cm mixed adenocarcinoma. The tumor showed EGFR protein overexpression identified by IHC and chromosome 7 high polyssomy by FISH.

The authors call attention to the fact that although EGFR and KRAS mutations are almost always mutually exclusive in some cases they may coexist in the same neoplasia.

Authors’ Affiliations

Instituto de Anatomia Patológica, Faculdade de Medicina da Universidade de Coimbra, Coimbra, Portugal
Centro de Investigação em Meio Ambiente, Genética e Oncobiologia (CIMAGO), Coimbra, Portugal
Centro de Pneumologia, Faculdade de Medicina da Universidade de Coimbra, Coimbra, Portugal
Serviço de Anatomia Patológica dos Hospitais da Universidade de Coimbra, Coimbra, Portugal


© Sousa et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.