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BMC Proceedings

Open Access

EGFR/erb-1, HER2/erb-2, CK7, LP34, Ki67 and p53 expression in preneoplastic lesions of bronchial epithelium

  • Lina Carvalho1, 2, 3, 4Email author,
  • Joana E Santo1,
  • Ana Alarcão1, 2,
  • Patricia Couceiro1, 2,
  • Maria R Silva1, 2, 3,
  • Ana Gomes2, 4,
  • Maria J d’Aguiar1,
  • Lia Teixeira1 and
  • Vitor Sousa1, 2, 3, 4
BMC Proceedings20104(Suppl 2):P64

Published: 24 September 2010

A prognostic interpretation of preneoplastic lesions would have impact in bronchial carcinoma early diagnosis and through the study of Erb-B family receptors as they have an important role in lung carcinogenesis. The existence of drugs as tirosine kinase inhibitors (TKis) stressed the importance of studying gene alterations for selected chemoprevention schemes and characterization of carcinogenesis.

Bronchial preneoplastic lesions were characterized by immunohistochemistry using the antibodies LP34 (high weigh molecular cytokeratin), CK7, Chromogranin A, Ki67, p53, C-erbB-2 and EGFR. HER2 and EGFR gene copy number was also evaluated by fluorescent in situ hybridization (FISH) in those lesions.

The expected results defined the origin cell for basal cell hyperplasia and squamous metaplasia as adaptative lesions and dysplasia. By known experiences and published data, beyond the stem cell, the spectral evolution of bronchial preneoplastic lesions was demonstrated by characterizing basal cells (LP34) and their neoplastic potentiality.

Dysplasias showed a higher expression of EGFR, Ki67 and p53 with a stepwise increase with the gravity of the respective grading. C-erbB-2 immunohistochemical overexpression was a rare event in preneoplastic lesions. Polysomy was the main mechanism for EGFR and HER2/neu higher gene copy number and together with increased proliferation index (Ki67) will account to preview bronchial carcinogenesis.

Authors’ Affiliations

Instituto de Anatomia Patológica – Faculdade de Medicina da Universidade de Coimbra, Coimbra, Portugal
Centro de Investigação em Meio Ambiente, Genética e Oncobiologia (CIMAGO), Coimbra, Portugal
Centro de Pneumologia – Faculdade de Medicina da Universidade de Coimbra, Coimbra, Portugal
Serviço de Anatomia Patológica dos Hospitais da Universidade de Coimbra, Coimbra, Portugal


© Carvalho et al; licensee BioMed Central Ltd. 2010

This article is published under license to BioMed Central Ltd.