Skip to main content

Evaluation of deletion of nuclease genes cluster in L. major

The nuclease is a surface enzyme unique to trypanosomatid parasites. These organisms lack the pathway for de novo purine biosynthesis and thus are entirely dependent upon their hosts to supply this nutrient for their survival, growth, and multiplication. There is a cluster on chromosome 30 which carries 2 copy of nuclease genes and 5 identical nuclease like proteins in L. major which are in cis form with 700-800 bp intergenic regions which have more than 80% homology. These data shows that this enzyme might play an important role in facilitating the survival, growth, and development of this important human pathogen. In previous studies, have been shown that L. major 3′NT/NU which is expressed specifically in promastigotes is not the key molecules involved in host purine salvaging pathway and thus in better understanding parasite strategies adopted to survive in sandflies. Therefore, only deletion of nuclease genes followed by sandfly infections experiments will allow determining its precise role in purine salvaging and sandfly infection and specificity. We have developed nuclease cluster heterozygote and homozygote knockout mutants, with homologous recombination technique, to evaluate deletion effects on survival of parasite and infection.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Noushin Davoudi.

Rights and permissions

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Davoudi, N., Hejazi, M., Khodayari, Z. et al. Evaluation of deletion of nuclease genes cluster in L. major. BMC Proc 5, P39 (2011). https://doi.org/10.1186/1753-6561-5-S1-P39

Download citation

Keywords

  • Purine
  • Homologous Recombination
  • Intergenic Region
  • Infection Experiment
  • Knockout Mutant