Skip to main content


  • Lecture presentation
  • Open Access

Leishmania donovani promastigotes evade the antimicrobial activity of neutrophil extracellular traps

  • 1,
  • 2,
  • 1 and
  • 2
BMC Proceedings20115 (Suppl 1) :P40

  • Published:


  • Public Health
  • Antimicrobial Activity
  • Human Neutrophil
  • Subsequent Activation
  • Superoxide Production

Upon their recruitment to a site of infection and their subsequent activation, neutrophils release DNA and a subset of their granule content to form filamentous structures, known as neutrophil extracellular traps, which capture and kill microorganisms. In this study, we show that Leishmania promastigotes induced the rapid release of neutrophil extracellular traps from human neutrophils and were trapped by these structures. The use of Leishmania mutants defective in the biosynthesis of either lipophosphoglycan or GP63 revealed that these two major surface promastigote virulence determinants were not responsible for inducing the release of neutrophil extracellular traps. We also demonstrate that this induction was independent of superoxide production by neutrophils. Finally, in contrast to wild type L. donovani promastigotes, mutants defective in lipophosphoglycan biosynthesis were highly susceptible to the antimicrobial activity of neutrophil extracellular traps. Altogether, our data suggest that neutrophil extracellular traps may contribute to the containment of L. donovani promastigotes at the site of inoculation, thereby facilitating their uptake by mononuclear phagocytes.

Authors’ Affiliations

INRS- Institut Armand-Frappier, Laval, Québec, Canada, H7V 1B7
Department of Medical Genetics, University of British Columbia, Vancouver, BC, Canada, V6H 3Z6


© Gabriel et al; licensee BioMed Central Ltd. 2011

This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.