Volume 5 Supplement 1
Effect of LPS, dsRNA or interferons on the phagocytosis of dying cells or mycobacteria by macrophages
© Vanzembergh et al; licensee BioMed Central Ltd. 2011
Published: 10 January 2011
Infection with intracellular pathogens can trigger a panel of innate immune responses including cell death. Coupled with phagocytosis this often leads to clearance of the invader. However, some pathogens use the process to disseminate and proliferate. During Mycobacterium marinum infection, dying infected macrophages recruit fresh ones to the site of granuloma formation. The recruited macrophages phagocytose infected cell remnants, get infected and die, thus ensuring efficient spread and multiplication of the pathogen. Maturation of granuloma, the characteristic lesions of tuberculosis, requires tumor necrosis factor (TNF) and interferon (IFN)-gamma and represents a stalemate between host and pathogen sufficient to arrest infection without eliminating the bacteria. Indeed, our macrophage / mycobacterial infection model demonstrates that virulent H37Rv Mycobacterium tuberculosis is more efficient in macrophage infection and killing than the attenuated Mycobacterium bovis BCG vaccine.
Today it is still unclear whether the signal promoting macrophage engagement in phagocytosis is originating from a pathogen or the infected host, and whether the effect is general or target specific. Therefore, we used a quantitative cell line based assay to study the effects of PAMPs or cellular alarm signals on the phagocytic engagement and capacity of macrophages to engulf virulent or attenuated mycobacteria and dying cells. Our results demonstrate that pretreatment of macrophage like cells with double stranded RNA, lipopolysaccharide, type I or II IFN but not with TNF, can significantly increase their capacity to phagocytose apoptotic and necrotic cells but has little effect on the phagocytosis of free mycobacteria. Although this macrophage activation process is probably an innate immune response reinforcing the capacity of the host to dispose of dying infected cells, pathogens may exploit it for their propagation.
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.