Innate antiviral immunity is impaired in young patients with hand foot and mouth diseases
- Y Yang1
© Yang; licensee BioMed Central Ltd. 2011
Published: 29 June 2011
Introduction / objectives
This study was designed to explore the expressions of three pattern-recognition receptors (Toll-like receptor 3, retinoic acid inducible gene-I and melanoma differentiation-associated gene 5) and components of their signaling pathways in the peripheral blood mononuclear cells of children patient with Hand, foot and mouth disease.
98 HFMD patients (aged of 1-5 years) and 55 age-matched non-infection children were enrolled in this study; the patients were divided into two groups according to clinical characteristics - with or without complications. The expressions of TLR3, RIG-I, MDA5, IRF-1 and IFN-alpha mRNA were detected by Real-Time PCR.
The expression levels of TLR3 mRNA in HFMD patients were significantly reduced (6.05±1.26) compared with the non-infection children (7.05±0.96), P<0.001, and the furthermore decreased was found in the patients with complications (5.79±1.15). While, the expressions of MDA5 mRNA in all patients including without complications (4.64±0.49) and with complications (4.60±0.48) were markedly higher than the non-infection children (4.16±0.35), P<0.001. However, RIG-I mRNA was detected only in 72/98 patients, which was not found in the non-infection children. IFN-alpha was lower in the patients without complications (5.71±1.26) than the non-infection children (6.19±0.86), and significantly decreased IFN-alpha mRNA transcriptions were found in the patients with complications (5.54±1.18), compared with the non-infection children P<0.05. Moreover, the changes of IRF-1 mRNA were similar with IFN-alpha, an evidently reduced level of IRF-1 was in the patient with complications (4.89±0.66) compared with the non-infection children (5.32±0.64), P=0.001.
It is suggested that innate antiviral immunity is impaired in patients and is possibly correlated with the severity of illness.
Disclosure of interest
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.