Figure 1

HA N -glycosylation patterns of Influenza A virus Uruguay/716/2007 ( H3N2 ) – high growth reassortant. Relative fluorescence units (RFU) are plotted over the migration time (t_mig) in base pairs (bp). (A) MDCK cell-derived virus (seed) was consecutively passaged in Vero cells (1^st passage to 5^th passage). Peaks annotated in blue are present in MDCK as well as Vero cell-derived HA; peaks annotated in black are MDCK cell-specific, and red annotation indicates Vero cell-specific peaks. (B) MDCK cell-derived virus harvested either 24 or 72 hours post infection (hpi). MDCK cell-specific peaks are annotated (1, 2, 4, 6 – 24, 26-28).