Table 1 Box-Behnken results. a Three levels of concentrations for each variable including a maximum (1), a minimum (-1) and a center point (0) were used. Values shown in parenthesis are concentrations employed for r-transferrin and r-insulin (mg/mL) and lipid mix (based on Synthechol® concentrations provided in X). b Cell density values presented are mean of duplicate runs in million cells/mL.
EXP N° | r-Insulina | r-Transferrina | Lipid Mixa | Max Cell densityb | |
|---|---|---|---|---|---|
Experimental | According to model | ||||
1 | -1 (1) | -1 (1) | 0 (1) | 3,1 | 2,8 |
2 | 1 (20) | -1 (1) | 0 (1) | 5,5 | 5,4 |
3 | -1(1) | 1 (20) | 0 (1) | 3,7 | 3,8 |
4 | 1 (20) | 1 (20) | 0 (1) | 3,3 | 3,7 |
5 | -1 (1) | 0 (10) | -1 (0.1) | 3,1 | 3,1 |
6 | 1 (20) | 0 (10) | -1 (0.1) | 4,6 | 4,4 |
7 | -1 (1) | 0 (10) | 1 (2) | 2,0 | 2,3 |
8 | 1 (20) | 0 (10) | 1 (2) | 3,6 | 3,6 |
9 | 0 (10) | -1 (1) | -1 (0.1) | 3,2 | 3,6 |
10 | 0 (10) | 1 (20) | -1 (0.1) | 3,5 | 3,4 |
11 | 0 (10) | -1 (1) | 1 (2) | 2,8 | 2,9 |
12 | 0 (10) | 1 (20) | 1 (2) | 2,8 | 2,4 |
13 | 0 (10) | 0 (10) | 0 (1) | 4,9 | 4,5 |
13 | 0 (10) | 0 (10) | 0 (1) | 4,9 | 4,5 |
13 | 0 (10) | 0 (10) | 0 (1) | 3,8 | 4,5 |