CAP-T cell expression system: a novel rapid and versatile human cell expression system for fast and high yield transient protein expression

Table 1 Table 1 summarizes the relevant data from transient transfections of CAP-T cells in different scales with plasmids coding for different proteins of interest (hAAT, erythropoietin (EPO), C1-Inhibitor and IgG). Cells were either transfected by nucleofection (1 x 10⁷ cells) or by PEI (1.7 x 10⁹ cells).

	hAAT		EPO	C1-Inhibitor	IgG
cells transfected	1 x 10⁷	1.7 x 10⁹	1 x 10⁷	1 x 10⁷	1 x 10⁷
culture volume [ml]	30	1000	60	30	30
culture time [days]	9	6	10	12	6
viability at harvest [%]	85	70	80	87	80
volumetric productivity [mg/L]	170	180	38	35	150

ISSN: 1753-6561