Table 1 Growth and protein production data of CHO producer clones in different assay formats
From: Toolbox approach for fast generation of stable CHO production cell lines from different hosts
| Â | Â | Fc-protein | Glycoprotein | ||
|---|---|---|---|---|---|
Assay format | Cell line | Max VCD [viable cells/mL] | Max Titer [mg/L] | Max VCD [viable cells/mL] | Max Titer [mg/L] |
96-well plate assay | K1 | nda | 50 | nda | 268 |
(5 days) | DG44 | nda | 176 | nda | 607 |
Batch | K1 | 2.3E+07 | 610 | 1.1E+07 | 1772 |
(5 or 7 days) | DG44 | 8.6E+06 | 980 | 6.4E+06 | 2200 |
Fed batch | K1 | 3.0E+07 | 2500 | 1.9E+07 | 8100 |
(12-17 days) | DG44 | 1.7E+07 | 4500 | 3.0E+07 | 13200 |