Figure 1
Effect of inoculum concentration on aggregate concentration (A), aggregate size (B) and viability (C), evaluated using a double stain viability test: fluorescein diacetate (live, green); propidium iodide (dead, red). Cells were cultured in shake flasks with inoculum concentrations of 2 and 4x105 cell/mL. Error bars denote standard deviation of average of 3 independent experiments. *** indicates significant difference (P<0.001) in aggregate diameter by one-way ANOVA analysis with a Tukey’s post-hoc multiple comparison test.