Table 1 Advantages and drawbacks of ADCC effectors for the validation of a standardized ADCC assay
From: ADCC potency assay: increased standardization with modified lymphocytes
ADCC effector types | Advantages | Drawbacks |
|---|---|---|
Primary NK or PBMC, isolated from donors | Representative from the genetic diversity | Not convenient for standardization Requirement for donor genotyping Requirement to evaluate several donors for accurate comparison Exposure of operators to biohazard Significant ressources required (budget and time) for cell preparation |
Modified NK cell lines | Increased suitability for standardization | The NK activity may interfere with the measurement of the ADCC-related lysis Absence of functionally qualified batches of cells Absence of biosafety qualified batches of cells To be handled as a GMO |
CD16-transduced lymphocytes | Increased suitability for standardization Qualified batches available in a ready-to-use format Absence of NK activity-related background | To be handled as a GMO |