Figure 1

a: In situ micrographs of hybridoma cells. Left: Typical portrait taken in a cell culture with high viability (>90%) Right: Typical portrait taken in a cell culture with low viability (<30%). b: Corresponding greyvalue histograms of the respective cell 8 bit encoded portrait. 0 = white; 255 = black. c: Viability signals of a Jurkat batch culture as determined by ISM (as PLV-signal), ViCell (automatic counting via Trypan blue exclusion) and Flow Cytometry (AnnexinV/FITC / PI assay). The culture was insulted with EtOH (final conc. 3%) after 43 h to assess viability measurement capabilities under difficult circumstances. Similar results were obtained after addition of Etoposide (final conc. 10µM), infliction of osmotic shock or no culture insult at all.