Table 1 Operating conditions of preparative chromatographic fractionations.
From: Characterization of chromatographic yeast extract fractions promoting CHO cell growth
Separation | Gel / Column | Fractions |
|---|---|---|
Anion-exchange | Q Sepharose, H 10 cm, ϕ 10 cm | A.1, A.2, A.3 |
Hydrophobic interaction | Phenyl Sepharose , H 10 cm, ϕ 5 cm | H.1, H.2 |
Size-exclusion | G-15 Sephadex, H 75 cm H, ϕ 5 cm | W.1, W.2, W.3 |
Sequentially three step process | same than one step processes | A.x-H.y-W.z |