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Volume 6 Supplement 6

Beyond the Genome 2012

A fast solution to NGS library preparation with low nanogram DNA input

Next-generation sequencing (NGS) has significantly impacted human genetics, enabling a comprehensive characterization of human genome as well as better understanding of many genomic abnormalities. By delivering massive DNA sequences at unprecedented speed and cost, NGS promises to make personalized medicine a reality in the foreseeable future. To date, library construction with clinical samples has been a challenge, primarily due to the limited quantities of sample DNA available. To overcome this challenge, we have developed a fast library preparation method using novel NEBNext reagents and adaptors, including a new DNA polymerase that has been optimized to minimize GC bias. This method enables library construction from an amount of DNA as low as 5 ng, and can be used for both intact and fragmented DNA. Moreover, the workflow is compatible with multiple NGS platforms.

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Correspondence to Pingfang Liu.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Liu, P., Lohman, G.J., Cantor, E. et al. A fast solution to NGS library preparation with low nanogram DNA input. BMC Proc 6, P26 (2012). https://doi.org/10.1186/1753-6561-6-S6-P26

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Keywords

  • Library Construction
  • Personalized Medicine
  • Library Preparation
  • Comprehensive Characterization
  • Fast Solution