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Volume 6 Supplement 6

Beyond the Genome 2012

  • Poster presentation
  • Open Access

A fast solution to NGS library preparation with low nanogram DNA input

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BMC Proceedings20126 (Suppl 6) :P26

https://doi.org/10.1186/1753-6561-6-S6-P26

  • Published:

Keywords

  • Library Construction
  • Personalized Medicine
  • Library Preparation
  • Comprehensive Characterization
  • Fast Solution

Next-generation sequencing (NGS) has significantly impacted human genetics, enabling a comprehensive characterization of human genome as well as better understanding of many genomic abnormalities. By delivering massive DNA sequences at unprecedented speed and cost, NGS promises to make personalized medicine a reality in the foreseeable future. To date, library construction with clinical samples has been a challenge, primarily due to the limited quantities of sample DNA available. To overcome this challenge, we have developed a fast library preparation method using novel NEBNext reagents and adaptors, including a new DNA polymerase that has been optimized to minimize GC bias. This method enables library construction from an amount of DNA as low as 5 ng, and can be used for both intact and fragmented DNA. Moreover, the workflow is compatible with multiple NGS platforms.

Authors’ Affiliations

(1)
New England Biolabs, Ipswich, MA 01938, USA

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